
NAD+ and AHK-Cu in Metabolic and Longevity Research: A Deep Dive
A research overview of NAD+ and AHK-Cu covering mitochondrial metabolism, sirtuin activation, copper-peptide signaling, and laboratory handling protocols.

AHK-Cu Spray is Veracue’s spray-dispensed format of AHK-Cu, the copper(II) complex of the tripeptide alanine-histidine-lysine, also catalogued as Copper Tripeptide-3. It differs from its better-known relative GHK-Cu by a single amino acid at the first position, and its reported molecular weight depends on which form a source describes, since several related representations circulate under the same CAS number, 682809-81-0. Confirm the exact form on a lot’s own documentation before relying on any single mass figure. Offered in 50 mg and 100 mg for laboratory research use only.
Ask for the analytical documentation of any lot.
HPLC is the method used to measure purity
Ask for the report for a specific lot through the contact page
Supplied for laboratory research use only
Mass spectrometry is the method used to confirm identity
AHK-Cu Spray is Veracue’s spray-dispensed packaging of AHK-Cu, a copper(II) complex of the tripeptide alanine-histidine-lysine. The underlying molecule is identical to the one documented on Veracue’s AHK-Cu vial listing; only the packaging and dispensing format differ between the two listings.
"Spray" is a packaging and dispensing descriptor, nothing more. It does not establish a route, a concentration, or a formulation, and none of those specifics should be inferred from the product name. Molecular form, formula, concentration and storage conditions are reported on each lot’s own documentation and can be requested through the contact page.
| Product name | AHK-Cu Spray (compound also listed as Copper Tripeptide-3) |
| Packaging format | Spray-dispensed, Research Use Only |
| Peptide sequence | Ala-His-Lys (A-H-K), three amino acids |
| Metal | Copper(II) |
| Closest relative | GHK-Cu (Gly-His-Lys copper), differing only in the first amino acid |
| Molecular weight | Form-dependent, see the molecular form table |
| CAS Registry Number | 682809-81-0 (widely listed; see note below) |
| Sizes offered | 50 mg and 100 mg |
| Research designation | Research Use Only |
Veracue lists AHK-Cu in more than one packaging format because research groups source and track material differently depending on how it is packaged. Keeping the formats as separate listings keeps lot records, images and documentation specific to the packaging a researcher actually ordered, rather than mixing records across formats.
AHK-Cu Spray and Veracue’s AHK-Cu vial listing describe one molecule: the tripeptide alanine-histidine-lysine bound to a copper(II) ion through nitrogen atoms of the alanine terminus and the histidine residue. Packaging format has no bearing on sequence, formula or mass, so these values are identical across both listings.
No. The compound reported under this listing is the same AHK-Cu reported under the vial listing. What differs between the two listings is how the material is packaged and dispensed, not its chemical identity.
There is no single molecular weight for AHK-Cu that applies to every product, regardless of packaging. Published values differ because sources describe different forms: the free peptide, the copper complex written with different proton counts, or a hydrochloride salt. The table below, carried over unchanged from the vial listing, shows how each common representation produces a different number.
| Representation | Formula | Molecular weight (g/mol) | Where you will see it |
|---|---|---|---|
| Uncomplexed tripeptide (AHK, no copper) | C15H26N6O4 | 354.41 | Peptide-only calculations |
| Neutral Cu(II) complex, two N-H protons removed | C15H24CuN6O4 | 415.94 | Some catalogue listings (about 416) |
| Cu(II) complex written with one additional proton | C15H25CuN6O4 | 416.95 | Common "about 416.9" listing value |
| Hydrochloride form, PubChem CID 168431292 formula | C15H24ClCuN6O4 | 451.39 | PubChem and several supplier pages |
| Hydrochloride form, alternative hydrogen count | C15H25ClCuN6O4 | 452.40 | Some chemical supplier listings |
The molecular form, formula and molecular weight for a given lot of either packaging format are reported on that lot’s documentation and can be requested through the contact page.
In Veracue’s catalog, "Spray" is a packaging and dispensing term, comparable to "vial" on other listings. It tells a researcher how the product is dispensed from its container. It does not specify a concentration, a carrier, a route, or a formulation, and it is not shorthand for any particular method of use. Those details, where applicable, live in lot-specific documentation rather than in the catalog name.
Treating a packaging word as a technical specification is a common source of error when comparing listings across suppliers. A researcher documenting materials in a methods section should cite the lot’s own documentation for format-specific details rather than inferring them from the word "Spray" alone.
| Attribute | AHK-Cu | GHK-Cu |
|---|---|---|
| Sequence | Ala-His-Lys + Cu(II) | Gly-His-Lys + Cu(II) |
| Common ingredient name | Copper Tripeptide-3 | Copper Tripeptide-1 |
| First amino acid | Alanine | Glycine |
| Catalog formats Veracue offers | Vial and spray | Vial and spray |
This table compares molecular structure and packaging options only. Catalog format is listed separately because it is a packaging detail, not a property of the molecule itself, and findings reported for one compound should not be assumed to apply to the other.
Typical contexts are cell-culture and tissue-culture work, copper-peptide chemistry and analytical method development. The material is offered for in vitro and laboratory research only, in either packaging format Veracue carries.
Because "Spray" and "vial" describe packaging rather than chemistry, a methods section should still name the molecule precisely by sequence, formula or CAS number, and separately note the catalog format and lot number actually used. This page provides molecular reference information and general research context only, and Veracue provides no dosing, administration or usage guidance of any kind.
Identity and purity for AHK-Cu Spray are established the same way as for any AHK-Cu lot: reverse-phase HPLC for chromatographic purity, and mass spectrometry (typically ESI-MS or LC-MS) to confirm that the observed mass matches the molecular form a report claims. HPLC alone shows that most detected material elutes together; it does not by itself confirm identity, and it does not establish copper content.
Packaging format has no bearing on which analytical methods apply. A spray-dispensed lot and a vial lot of the same molecule are verified against the same sequence and molecular-weight references, listed on the AHK-Cu at a Glance table above.
| Method | Tells a researcher | Does not prove by itself |
|---|---|---|
| RP-HPLC | Chromatographic purity (area %) | Identity; copper content |
| ESI-MS / LC-MS | Mass consistent with a stated AHK-Cu form | Which species was intended; purity |
| Copper content assay (if tested) | Whether copper is present at the expected ratio to peptide | Purity of the peptide portion |
| Net peptide content | How much of a lot’s mass is peptide rather than salts or water | Chromatographic purity |
A certificate of analysis describes one lot, never a packaging format or a compound in general. Before relying on any figure, confirm that the lot number on the certificate matches the container and the order record for the spray-format unit received.
A complete certificate separates product identity, lot number, stated molecular form, HPLC result, mass spectrometry result with observed and theoretical mass, test date and testing laboratory. The certificate page explains how to request lot documentation for either format.
No. The verification question is the same: does this lot match the stated molecular form, and is the purity figure paired with a mass result from the same lot. Packaging format does not change what counts as adequate documentation.
AHK-Cu can be reported as the free peptide, the neutral copper complex, or a hydrochloride salt, and these forms carry different formula weights, from 354.41 up to 452.40 g/mol. If a lot’s documentation leaves the form unstated, any mass-based calculation can carry a silent error. Confirm the form on the lot’s certificate and use the matching formula weight, regardless of packaging format.
For each lot, keep the catalog listing snapshot, the lot-specific certificate, the stated molecular form, the receipt date, labeled storage conditions, and the packaging format actually received. Recording format alongside the usual identity fields keeps multi-format catalogs like this one traceable lot to lot.
AHK-Cu Spray is supplied for laboratory research and analytical characterization only. It is not intended for human or veterinary use, or for ingestion, injection or any form of administration. This page contains no administration or usage guidance of any kind, and the word "Spray" in the product name describes Veracue’s packaging and dispensing format only. Veracue provides no dosing guidance for this or any research material. See the Medical Disclaimer and the Terms and Conditions for sitewide policies.

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