# Thymosin Alpha-1 Spray

> Research use only. Not for human or veterinary use. Supplied by Veracue Labs.

- Page: https://veracuelabs.com/product/thymosin-alpha-1-spray
- Category: Nasal Sprays
- Sizes and prices in USD: 10 mg at $93.49
- Certificate of analysis: none posted on the page. Request one for a specific lot at https://veracuelabs.com/contact-us.
- Prices are a snapshot from 2026-10-16. The product page shows the current price.

## Overview

Thymosin Alpha-1 Spray is a spray-dispensed form of thymosin alpha-1, a linear 28-amino-acid peptide with an acetylated N-terminal serine and the sequence Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH. Reference values for the peptide are formula C129H215N33O55, average mass 3108.3 g/mol and CAS 62304-98-7. The chain is the opening 28-residue stretch of prothymosin alpha, and it is a different molecule from thymosin beta-4 (43 residues) and from TB-500, a seven-residue piece of beta-4.

## Product Details

### Thymosin Alpha-1 Spray Specifications

Product name: Thymosin Alpha-1 Spray (the peptide is also written Tα1 or TA1)
Format: Spray-dispensed bottle
Sprays per bottle: 45 sprays per bottle
Size offered: 10 mg
Chain: 28 amino acids, linear, N-terminal serine acetylated, free C-terminal acid
Sequence (one-letter code): Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH
Sequence (three-letter code): Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH
Origin of the sequence: Residues 1 to 28 of prothymosin alpha
Molecular formula: C129H215N33O55
Average mass: 3108.3 g/mol
Monoisotopic mass: 3106.50 Da
CAS Registry Number: 62304-98-7
PubChem CID: 16130571
Research designation: Research Use Only

Formula, mass and CAS number describe the peptide as recorded in chemical databases and are not measured results for any bottle. Chemical form, concentration, fill volume and carrier are not stated on the listing. Certificates of analysis are not published on the site yet; documentation can be requested through the contact page for particular products.

### The Peptide Behind the Spray

The material in the bottle is thymosin alpha-1, a synthetic chain of 28 amino acids. Read from the N-terminus it is serine, aspartic acid, two alanines, valine, aspartic acid, and so on through to a final asparagine. The serine at position 1 carries an acetyl group, and the asparagine at position 28 ends in a free carboxylic acid.

Those 28 residues are the opening stretch of the larger protein prothymosin alpha, which makes the peptide a fragment of that protein. The full precursor is not what is sold here. The listing is one defined molecule at one size, 10 mg, in a bottle that holds 45 sprays.

### Names That Look Alike

- Thymosin beta-4 is a separate, longer peptide of 43 residues.
- TB-500 is generally described as the seven-residue fragment Ac-LKKTETQ taken from thymosin beta-4, with the formula C38H68N10O14, an average mass near 889 g/mol and CAS 885340-08-9.
- Prothymosin alpha is the larger precursor protein from which the 28 residues derive.
- Not a dietary, cosmetic or other consumer item, and not for use in people or animals.

A purchasing record that says only thymosin cannot tell these apart. The residue count, 28 against 43 against 7, settles the question at once.

## Research Focus & Mechanism Overview

### What the Sequence Tells You

### Which residues are present, and which are missing?

Counting the chain gives three each of serine, aspartic acid, alanine, valine and threonine, six glutamic acids, four lysines, and one each of isoleucine, leucine and asparagine.

Nine residues are acidic (three Asp and six Glu) against four lysines, which is why the peptide is strongly acidic, with a reported isoelectric point of about 4.0 to 4.3. The lysines cluster between positions 14 and 20, at positions 14, 17, 19 and 20.

What is absent matters as much. There is no cysteine or methionine, so no disulfide bond and no sulfur anywhere in the formula, and there is no tryptophan, tyrosine or phenylalanine, so the chain has no aromatic side chain at all.

### What does the acetyl group do to the numbers?

Acetylation adds C2H2O, about 42 Da, to the chain. The same 28 residues with a free amine at the N-terminus would be C127H213N33O54 and average roughly 3066.3 g/mol, so a record quoting that figure describes the non-acetylated chain and not thymosin alpha-1.

### Thymosin Alpha-1, Thymosin Beta-4 and TB-500 Side by Side

Feature: Thymosin alpha-1: Thymosin beta-4: TB-500

Length: 28 residues: 43 residues: 7 residues
Relationship: Fragment of prothymosin alpha: Parent protein of TB-500: Residues 17 to 23 of thymosin beta-4
Sequence: Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH: Full 43-residue chain: Ac-LKKTETQ
Average mass: 3108.3 g/mol: Higher than either, with 43 residues: About 889 g/mol
CAS number: 62304-98-7: Not listed here: 885340-08-9

These are three separate molecules. The shared word in the name is a naming convention and not a shared structure, so a finding reported for one does not carry over to another.

### Assay-Level Receptor Context

Reports in cultured dendritic cells and related innate-immune cell types point to Toll-like receptor pathways, chiefly TLR2 and TLR9. Typical readouts are maturation markers and the cytokines IL-2 and IFN-γ.

Each result belongs to its assay. The cell type, the stimulus and the form of the peptide all shape what is seen, so a reading from one system describes that system only. Veracue gives no dosing, administration or usage guidance of any kind.

### Questions a Methods Section Should Answer

### How should the peptide be named?

By sequence or formula first and common name second, for example Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH or C129H215N33O55, with registry number 62304-98-7 as a cross-check. The common name alone, whether thymosin alpha-1, Tα1 or TA1, leaves room for confusion with other thymosins.

### What else should be recorded?

The chemical form, the lot identifier on the bottle, the measured mass and the date of receipt. For a spray-dispensed product, add the count of 45 sprays per bottle, and note that concentration, fill volume and carrier are not stated on the listing.

## Quality & Purity Standards

### Mass Spectrometry of a 3.1 kDa Acidic Peptide

Electrospray of a peptide near 3108 g/mol gives a series of multiply charged ions and not a single peak at the neutral mass. Thymosin alpha-1 has only four lysines and an acetylated N-terminus, so positive mode favors low charge states, while its nine acidic residues allow ions in negative mode as well.

Calculated average-mass values for the ions most often inspected:
- Positive mode: 3+ near m/z 1037.1 and 4+ near m/z 778.1.
- Negative mode: 4- near m/z 776.1 and 5- near m/z 620.7.

Multiply each m/z by its charge and correct for the proton mass, and every peak in a series should return the same neutral value, near 3108.3 g/mol. Instruments that report monoisotopic masses read about 1.8 Da lower, near 3106.50, so check which convention a report uses.

### Reading the Spectrum

What the spectrum shows: A reasonable reading

Peaks that all convert to one neutral mass near 3108.3: One species seen in several charge states
A main mass about 42 Da lower, near 3066.3: The acetyl group is missing, so the chain has a free N-terminus
A main mass lower by one residue mass, such as 71.1 (Ala), 87.1 (Ser) or 129.1 (Glu): A deletion species missing one residue from the chain
Peaks about 22 Da above the main mass: Sodium adducts, which move observed values without changing the molecule
A main mass far from 3108.3: A different peptide, a modified chain or another chemical form

### Quantifying a Peptide With No Aromatic Residues

Tryptophan and tyrosine supply most of a peptide’s absorbance at 280 nm, and thymosin alpha-1 contains neither. A concentration estimate read at 280 nm has nothing to measure, and a calculation that assumes a typical extinction coefficient does not hold for this chain. Peptide-bond absorbance at shorter wavelengths or amino acid analysis is the usual route, and net peptide content shows how much of a weighed mass is peptide as opposed to salt or water.

### Documentation for a Bottle

Certificates of analysis are not published on the site yet; documentation can be requested through the contact page for particular products. A document describes one lot and nothing else, so the first check is that its lot number matches the bottle label and the order record.

When a document is supplied, useful fields are the chemical form, an observed mass set against the expected 3108.3 g/mol, a chromatographic purity result with its method named, net peptide content, fill volume and concentration, the test date and the testing laboratory. A purity figure with no method or lot number attached cannot be checked.

The figure of 45 sprays per bottle is a packaging count. It says nothing about peptide content per spray, and no purity value, testing method or storage condition is stated for the product on the listing.

### Verification Questions

### Does a clean chromatogram confirm that a lot is thymosin alpha-1?

No. A chromatogram shows how much of the detected signal sits in one peak, and a related peptide of similar polarity could give an equally tidy trace. The mass result and, where needed, fragment data across the N-terminus carry the identity.

### Why does the chemical form matter for a mass-based calculation?

The reference numbers here describe the free peptide, and a counterion adds weight on top of it. Weighing out or comparing masses without knowing which form is present builds in an offset.

## Compliance Notice

### Research Use Only Statement

Thymosin Alpha-1 Spray is a laboratory reagent, sold for research and analytical work alone. It is not a drug, dietary supplement, cosmetic or food, and it is meant for no human or veterinary use in any form. Veracue provides no dosing, administration or usage guidance of any kind for this or any other research material. Buyers must be qualified researchers or institutions and are responsible for lawful handling. Sitewide terms are set out in the Medical Disclaimer and the Terms and Conditions.

## Questions and answers

### How many sprays does one bottle hold, and what strength is offered?

Each bottle holds 45 sprays, and the one size offered is 10 mg. The spray count is a packaging fact, while concentration and fill volume are not stated on the listing.

### What peptide is in Thymosin Alpha-1 Spray?

A single synthetic peptide, thymosin alpha-1: 28 amino acids with an acetylated N-terminal serine, formula C129H215N33O55 and CAS 62304-98-7. It is also written Tα1 or TA1.

### Why does the sequence begin with Ac-?

Ac marks the acetyl group on the N-terminal serine. Acetylation adds about 42 Da, so the same chain without it would average near 3066.3 g/mol instead of 3108.3.

### Which amino acids does thymosin alpha-1 not contain?

It has no cysteine, methionine, tryptophan, tyrosine, phenylalanine, arginine, histidine, proline or glycine. That means no disulfide bonds, no sulfur in the formula and no aromatic absorbance at 280 nm.

### What mass should a lot of thymosin alpha-1 show?

About 3108.3 g/mol on an average basis, or 3106.50 Da monoisotopic. Electrospray gives ions such as the 4+ peak near m/z 778.1 in positive mode, and a salt form carries extra mass.

### What does a signal about 42 Da below the expected mass suggest?

The usual explanation is a chain that lacks its acetyl group. A lighter signal equal to one residue mass, such as 71.1 for alanine, points to a deletion instead.

### Is TB-500 another name for thymosin alpha-1?

No. TB-500 is generally described as the seven-residue fragment Ac-LKKTETQ from thymosin beta-4, with CAS 885340-08-9 and a mass near 889 g/mol, so it shares neither length nor identifiers with this peptide.

### What separates thymosin alpha-1 from thymosin beta-4?

Thymosin beta-4 is a 43-residue peptide, while thymosin alpha-1 has 28 and comes from prothymosin alpha. The shared word is a naming convention, so the residue count is the quickest way to tell them apart.

### How does thymosin alpha-1 relate to its precursor protein?

Its 28 residues are the opening stretch of prothymosin alpha, which makes it a piece of that larger protein and not an alternative name for it.

### Why can a 280 nm reading not be used to quantify it?

The peptide contains no tryptophan or tyrosine, the residues that supply most absorbance at that wavelength. Quantification relies on peptide-bond absorbance at shorter wavelengths or on amino acid analysis.

### Can a registry number alone confirm identity?

It is a useful cross-check, and 62304-98-7 with PubChem CID 16130571 are the identifiers used here, but records occasionally carry a different number for the same name. Sequence and mass are the firmer test.

### What cell systems appear in laboratory reports on thymosin alpha-1?

Reports describe dendritic cells and other innate-immune cell types, Toll-like receptor pathways (chiefly TLR2 and TLR9) and readouts such as maturation markers, IL-2 and IFN-γ. Each finding belongs to its own assay, so none of them speaks for the peptide in general.

### Are certificates of analysis published for this product?

Not yet. Certificates are not published on the site, and documentation can be requested through the contact page for particular products.

### What does the research use only label mean for this bottle?

The contents are a laboratory reagent for analysis and research, not something for human or veterinary use.

### Is there usage guidance for Thymosin Alpha-1 Spray?

No. The bottle is offered for laboratory research only, and Veracue provides no dosing, administration or usage guidance of any kind.

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